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this opens voltage-dependent Ca 2+ channels, elicits action potentials, and establishes early network activity and excitation in the developing nervous system [68]

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The protein concentration was 10 mg/ml, while the peptide concentration was 5 mM (20:1 peptide:protein molar ratio)

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The lysate was centrifuged at 13,200 rpm for 10 min at 4C, and cleared lysate was used to determine the amount of GSH in the sample using the QuantiChrom glutathione assay kit (BioAssay Systems)